Custom DNA-RNA Synthesis:Purification | Hokkaido System Science Co.,Ltd.

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Purification

Gel Filtration (11 - 50mers)

Gel filtration takes off loose protecting group that comes off during the synthesis process. As a purification principle, it cannot clear out things with large molecular weight. Thus, synthesized DNA with defect base may remain. Also, short length oligos (less than 10 mer) may be resulted in low recovery rate.

Basically it is suitable for amplification of DNA fragment.

Reverse-phase Cartridge Purification (- 70mers)

This has higher purity than gel filtration. However, the resin nature may make its separation power decreased for long oligos. Thus, we recommend it for oligo shorter than 70 mers.

It is suitable for primers for sequencing or PCR for subcloning.

TOF/MS - Reverse-phase Cartridge Purification (- 35mers)

This is the same purification as above but confirmed identity by MALDI-TOF/MS analysis. For TOF/MS performance, this is a limited service for the product under 35mers.

HPLC Purification (- 120mers)

This is purification widely available for different purposes such as long oligos (over 30mers) or modified oligos. Specially, many of the impurities produced on the synthesis process can be taken off. Oligos shorter than 35mers are confirmed identity by MALDI-TOF/MS analysis after reverse-phase HPLC purification.

Pls. select this for high spec. experiment like mutagenesis or antisense.

IEX HPLC Purification (11 - 30mers)

Oligos can be separated by base with this purification method, which makes it easier to separate the base defective oligos produced on the synthesis process than normal HPLC purification. As reverse-phase HPLC purification, this also confirms identity with MALDI-TOF/MS analysis. For higher grade purification, it is recommended to apply this purification after reverse-phase HPLC purification. (For detail, please see the "High Purification DNA" section.)

PAGE Purification (- 150mers)

This highly purified oligo is purified by PAGE and then strictly quality controlled by MALDI-TOF/MS analysis (under 35mers).
It is suitable for long DNA or artificial gene, etc.

High Purity DNA (20 - 50mers)

Combined purifications are applied for this service: HPLC + IEX HPLC purifications for 20 - 30mers oligos, and HPLC + PAGE purifications for 31 - 50mers oligos. Such combined purifications enable average of 98% purity by taking off most of base defective products or other impurities. This is suitable for experiments which require highly purified oligos.

* MALDI-TOF/MS analysis is limited to product under 35 mers.

Guide for selecting purification grade
Here is the guide for selecting purification grade for your order.

	Gel Filtration	Reverse-phase Cartridge Purification	HPLC Purification	IEX HPLC Purification	PAGE Purification	High Purity DNA
Gene amplification	G	G	G	G	G	E
Sequencing primers	N	G	G	G	G	E
Gene amplification for subcloning *1	N	G/I	G	G	G	E
Degenerated DNA *2	N	G	N	N	N	N
Mutagenesis	N	N	G	G	E	E
Gene synthesis	N	N	G	I	E	I
Oligo longer than 51mers	N	G	E	N	E	N
Oligo longer than 71mers	N	N	G	N	E	N
Modification (terminal)	N	N	G	N	N	N
Modification (insertion)	N	I	G	N	N	N
Modification (Fluorescent)	N	N	G	N	N	N
Phosphorothioate Oligo (S-oligo)	N	N	G	N	N	N

E: Excellent
G: Good
I: Inquiry required (availability depends on sequence and modification)
N: Not recommended

*1 HPLC or PAGE purification are recommended for oligos longer than 30mers or if require large amount of clones.
*2 Degenerated DNA means oligos with mixed base insertion.

* Please note that this table is only a guide without guarantee for your experiment. If anything wrong with using our product, please contact us.