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Custom DNA - RNA Synthesis
Ordering

Yield & Domestic Order Lead time
Yield Guarantee Non-modified DNA
Purification Synthesis Scale Available Length Comments
-10mer 11-50mer 51-70mer 71-99mer 100-120mer 121-150mer
Gel Filtration
Reverse-phase cartridge purification		 OLIGOKIDS
(15nmol)*6		 - 1 OD - - - - Purification for OLIGO KIDS scale (15nmol) order is limited to gel filteration and reverse-phase cartridge purification.
Gel Filteration*1 25µmol - 2 OD - - - -  
0.05µmol - 5 OD - - - -
0.2µmol - 8 OD - - - -
1µmol - 30 OD - - - -
Reverse-phase cartridge purification*2

TOF/MS-Reverse-phase cartridge purification*3 		25µmol - 1 OD - - - -  
0.05µmol - 3 OD - - - -
0.2µmol 1 OD 5 OD 5 OD - - -
1µmol 5 OD 20 OD 20 OD - - -
HPLC purification 25µmol - 1 OD - - - - Degenerated base not available
0.05µmol - 2 OD - - - -
0.2µmol 1 OD 3 OD 2 OD 1 OD - -
1µmol 4 OD 12 OD 6 OD 2 OD 1 OD -
IEX HPLC purification*4 0.2µmol - 1 OD - - - - Available only in 0.2 µmol scale

Degenerated base not available
High Purification*5 1µmol - 1 OD - - - - Available only in 1µmol scale

Degenerated base not available
PAGE Purification 0.2µmol - 1 OD 1 OD 1 OD - - Available only in 0.2µmol and 1µmol scale

Degenerated base not available
1µmol - 4 OD 4 OD 2 OD 1 OD 1 OD
  * Purification available for oligos less than 10mers are reverse-phase cartridge, TOF/MS-reverse-phase cartridge and HPLC.
Also, for oligos between 71 and 120mers, HPLC or PAGE purification is available. For oligos between 121 and 150mers, only PAGE purification is available.
 
*1 Gel filtration is available for the oligos between 11 and 50mers.
*2 Reverse-phase cartridge purification is available for the oligos up to 70mers. HPLC purification or PAGE purification are recommended for the oligos longer than 71mers.
HSS will not be responsible for the orders other than this specification.
*3 TOF/MS data attachment is available only for the oligos up to 35mers.
*4 IEX HPLC purification is available only for the oligos between 11 and 30mers.
*5 High purification is available only for the oligos between 20 and 50mers.
*6 More than two oligos order is required for OLIGO KIDS (15nmol) scale products. Only one oligo order is dealt as 25nmol scale product.

Please click here for how to select the adequate purification.
Yield Guarantee Modified DNA
Purification Synthesis Scale Available Length Comments
Up to 10mer 11-50mer 51-70mer 71-99mer 100-120mer 121-150mer
Reverse-phase cartridge purification*1 0.2µmol - 1 OD 1 OD - - -  
1µmol - 8 OD 4 OD - - -
HPLC Purification 0.2µmol - 1 OD 1 OD 1 OD - - Degenerated base not available
1µmol*2 - 8 OD 4 OD 2 OD Pl. inquire. -
  * Orders for modified oligos are accepted in 0.2 µmol or more scale.
* For gel filtration and reverse-phase cartridge purification, modification is limited to middle of sequence or 3f-end except for phosphorothioate.
* Modified DNA is available only for HPLC purification except amination and phosphorylation which are available from Reverse-phase cartridge purification.
* Modification for 3'-end is available only for the oligos between 11 and 50mers.
* Phosphorothioated oligo is available from 1mg. Guaranteed yield for Texas Red DNA is total 50pmol.
 
*1 Reverse-phase cartridge purification is available for the oligos between 11 and 70mers.
*2 Guaranteed yield for 1µmol scale phosphorothioate oligo is 15 OD
 
  Please click here for detailed modification list.
Domestic Order Lead Time : Non-Modified DNA
    Order date is counted as "0" day. Sundays and Holidays are excluded.
* Delivery date for orders placed on Sundays and Holidays are set as of orders placed on the next business day.
Purification Available Length -10mers 11-50mers 51-70mers 71-99mers 100-120mers 121-150mers Comments
Gel filtration 11-50mers - 1 - - - -  
Reverse-phase cartridge column purification -70mers 2 1 2 - - -  
TOF/MS-Reverse-phase cartridge column purification*1 -35mers 3 2 - - - - TOF/MS data attachment is available only for the oligos up to 35mers.
HPLC purification -120mers 6 5 6-12 7-14 8-14 - Degenerated base not available
IEX HPLC purification*2 11-30mers - 6 - - - - Available only in 0.2µmol scale

Degenerated base not available
High purification*3 20-50mers - 10 - - - - Available only in 1µmol scale

Degenerated base not available
PAGE purification 11-150mers - 6 7-12 9-14 9-14 9-14 Available only in 0.2µmol or more scale

Degenerated base not available
  * For the orders of 1µmol or more scale, extra one day will be required to the above turnaround.
  * If the order is for large quantity or specific sequence, it may take longer for delivery.
  * HSS will not be responsible for the orders other than the available length in the above table.
  * Purification available for oligos less than 10mers are reverse-phase cartridge, TOF/MS-reverse-phase cartridge and HPLC.
Also, for oligos between 71 and 120mers, HPLC or PAGE purification is available, and for oligos between 121 and 150mers, only PAGE is available.
  *1 TOF/MS-Reverse-phase cartridge purification is available for the oligos up to 35mers.
*2 IEX HPLC purification is available only for the oligos between 11 and 30mers.
*3 High purification is available only for the oligos between 20 and 50mers.
  Please click here for how to select the adequate purification.
Domestic Order Lead Time : Modified DNA
    Order date is counted as "0" day. Sundays and Holidays are excluded.
* Delivery date for orders placed on Sundays and Holidays are set as of orders placed on the next business day.
Purification Available Length -10mers 11-50mers 51-70mers 71-99mers 100-120mers 121-150mers Comments
Reverse-phase cartridge column purification*1 11-70mers - 7-12 7-12 9-14 Please inquire. -  
HPLC purification 11-120mers Degenerated base not available
  * For the orders of 1µmol or more scale, extra one day will be required to the above turnaround.
* If the order is for large quantity or specific sequence, it may take longer for delivery.
* For Texas Red modification, it takes 7 business days.
* Modified DNA is available only for HPLC purification except amination and phosphorylation which are available from Reverse-phase cartridge purification.
* Modification for 3'-end is available only for the oligos between 11 and 50mers.
*1 Reverse-phase cartridge purification is available only for the oligos between 11 and 70mers.
Please refer to the Modification List for detail.
Domestic Order Lead Time : Large Amount DNA Synthesis
    Order date is counted as "0" day. Sundays and Holidays are excluded.
* Delivery date for orders placed on Sundays and Holidays are set as of orders placed on the next business day.
Yield Guarantee Non-Modified DNA Modified DNA Phosphorothioate DNA*1
1-2mg 7-12 Please inquire. 7-12
3-5mg 7-12 7-12
10mg 9-14 9-14
  * Purification available for miligram orders is only HPLC.
  * Please inquire on the oligo more than 10mg.
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