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Next Generation Sequencing Analysis Service
Service Description Applications Sample pre-treatment Analysis Principle
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Service Description

Hokkaido System Science Co., Ltd. provides high-throughput DNA sequencing service using
next generation sequencers, illumina "HiSeq 2000", "Genome Analyzer IIx" and Roche
"Genome Sequencer FLX (GS FLX)".
Various data spec is available from different systems. HiSeq2000 generates enormous data
volume of 200Gbp (equivalent to two human genome) per run, while Roche FLX provides long
read anaysis of 350~400 bp per read. Please choose the most suitable system for your
analysis purpose.

The analysis spec far larger than the conventional methods enables data sequencing from
mega to giga basis.
The broad applications are available from genomics to transcriptmics by utilizing sequencers
with different analysis principles.
We are qualified as a "Certified Service Provider" by Illumina, Inc. We offer a CSPro service which is certified through the strict review and training.
The sequencers used for analyses
As the amount of reads and data per one run is quite large, this service
is suitable for mutation analysis by re-sequencing and quantitative gene
expression analysis .

As the amount of reads and data per one run is quite large,
this service is suitable for mutation analysis by re-sequencing
and quantitative gene expression analysis .

  Click here for sequencing analysis principle.

Application
Re-sequencing ChIP sequencing
mRNA-seq small RNA analysis
Readable base numbers per 1 data: up to 400bp
Readable data numbers per 1 run: approx. 1 million
Readable base numbers per 1 run: approx. 400Mbp

Readable base length per 1 data is longer, suitable for novel genome analysis.

    Click here for sequencing analysis principle

e.g. development of industrial microorganisms, genome analysis of novel organisms

Application
De novo analysis
Target sequencing by NimbleGen
sequence capture

Readable base numbers per 1 data: 50bp/75bp
Readable data numbers per 1 run: approx. 100 million
Readable base numbers per 1 run:
Single read / approx. 5~7.5bp
Pair End / approx. 10~15bp

Larger quantity of data can be generated per each run, suitable for mutation analysis of existing genome and gene expression analysis.

    Click here for sequencing analysis principle.

e.g.: control of strains' mutation, expression analysis for drug discovery
Application
Re-sequencing ChIP sequencing
mRNA-seq small RNA analysis

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