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Hokkaido System Science Co., Ltd. (HSS) delivers sequence data with very short turnaround and at competitive prices. The sequencing is based on high quality oligo-synthesis technique.
HSS strives to work as each customer's own Sequencing Dept.
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Base sequence data of your DNA sample will be determined with your provided DNA sample and primer.
Quick and accurate analyses make the analysis report available within 2 working days.
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 Service Description
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Service Description |
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This service reports the sequence data from one sequencing reaction.
A standard sequence data comprise approximately 500 bases, however; the actual number of
bases may differ depending on the status of the sample.
Please select from the below services on your request.
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Pre-mix Service:
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One time sequencing service using your pre-mixed template and primer.
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One-pass Service:
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One time sequencing service with separate template and primer
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Full Service:
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Full sequencing service including sample QC and re-analysis
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The service includes;
- Agarose gel electrophoresis
- Sequencing reaction (one reaction)
- Sequencer run
- Data check
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Reagent: |
BigDye Terminator v3.1
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Equipment: |
PRISM 3130 Genetic Analyzer
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PRISM 3730 DNA Analyzer
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* Normally, the sequencing reaction is performed only once. However, the sequencing is
redone when the first try gives no result at all.
* If analytical difficulty such as GC rich and conformation is expected for ordering samples,
please let us know in advance.
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Deliverable |
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The report is to be sent via e-mail.
Deliverables
- base sequence data (text file)
- chromatochart (PDF file)
* Please inquire if you require Raw Data (ab1 file).
* For large numbers of Data,
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Price & Domestic Order Lead-time
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Price & Domestic Order Lead-time |
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Pre-mix |
One-pass |
Full Service |
| Price (tax exclusive) |
1,500 JPY / analysis |
2,000 JPY / analysis |
3,500 JPY / analysis |
| Domestic Lead-time |
Within 2 working days after the sample arrival |
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* Depending on sample quantity or analysis conditions, it may take longer for delivery.
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How to order
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How to order |
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Please download the order form, fill the blanks and send it attached to an e-mail to .
After sending the order form, please send your samples, primers, electrophoresis photo and order form to the below address.
Please clearly state as "sequencing samples" on the package.
Attn. Custom sequencing team
Hokkaido System Science Co., Ltd.
2-1, Shinkawa Nishi 2-1, Kita-ku, Sapporo-shi
Hokkaido, 001-0932
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* Please make sure that your package will be delivered on working days. Thank you for your
understanding.
* The client is to bear all the actual costs of shipping.
* Please read through the cautions on your order placement.
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Please provide: |
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DNA sample
(for 1 reaction) |
Plasmid: Concentration; 100-300ng/uL, Quantity; 10uL or more *1 |
| PCR products: Concentration; 10ng/uL or more, Quantity; 10uL or more *1,2 |
Primer
(custom primer) |
For any kind of sample: Concentration; 10uM, Quantity; 10uL or more
(For free-of-charge universal primers, please select from here.
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| Electrophoresis photo |
Please send a photo which shows the result of a marker and your sample on the same gel with their quantity description. |
| Order form |
Please download the form from our web site. |
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*1 Depending on size, higher sample concentration than the above may be required. Please
click here for the detail.
*2 For PCR products, please make sure to be purified after PCR reaction. For the sample
purification order to us, please click here for the detail.
*3 For the detailed information, please see at the below links.
- For sample preparation, click here.
- For required sample volume, click here.
- For electrophoresis photos, click here.
- For universal primer list, click here.
*4 Please read through the notice page before the actual order placement.
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Additional Sequencing |
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Additional sequencing, based on the result of the primary sequencing, can be performed on the same sample upon request. Please inquire if there is enough sample left, and send us another order sheet.
Due to the storage, samples used for sequencing analysis are destroyed after three working days from the analysis completion.
Please order for an additional sequencing within 3 working days after the primary sequencing report delivery.
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All rights reserved by Hokkaido System Science Co.,Ltd.
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