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Hokkaido System Science Co., Ltd. (HSS) delivers sequence data with very short turnaround and at competitive prices. The sequencing is based on high quality oligo-synthesis technique.
HSS strives to work as each customer's own Sequencing Dept.
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Long sequences are determined by Primer Walking method with your provided DNA samples.
High quality data is available by continuous analysis using primers designed and synthesized from the result of the previous sequencing.
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 Service Description
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Service Description |
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Several sequencing analyses are performed with your provided DNA sample.
New primers are designed from the sequencing result and synthesized to be used for another sequencing reaction, hence long sequences are determined by repeating this process.
There are two methods to choose from, Single Strand Sequencing (SSS) and Double Strand Sequencing (DSS), depending on the application.
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The service includes;
- Agarose gel electrophoresis
- Primer design and synthesis
- Sequencing reaction
- Sequencer run
- Data check
- Assemble data generation
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Reagent: |
BigDye Terminator v3.1
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Equipment: |
PRISM 3130 Genetic Analyzer
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PRISM 3730 DNA Analyzer
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* A standard sequence data comprise approximately 500 bases at one reaction. Repetitive
analyses can be performed to cover all bases required to be analyzed.
E.g.) For primer walking (SSS) of approx. 3.0kb sequence;
Normally, approx. 6 reactions will be performed. (3,000bp / 500bp per reaction = 6 reactions.)
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Deliverables |
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The report is to be sent via e-mail and CD-R.
Deliverables
- HSS synthesized primer
- CD-R
Data in CD-R
- Base sequence data (Text file)
- Chromatochart (PDF file)
- Assembled data
- Primer information
* Progress reports for each sequencing reaction can be delivered via e-mail upon request.
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Price & Domestic Order Lead-time
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Price & Domestic Order Lead-time |
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Price and Lead-time is changing depend on the required length. Below prices include primer design and synthesis, sequencing analysis and assemble.
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(tax exclusive)
| Length to be analyzed |
Single strand |
Lead-time |
Double strand |
Lead-time |
| Uo to 1.0kbp |
22,000 JPY |
Approx. 2 weeks |
44,000 JPY |
Approx. 2 weeks |
| Uo to 1.5kbp |
30,500 JPY |
Approx. 3 weeks |
61,000 JPY |
Approx.3 weeks |
| Uo to 2.0kbp |
39,000 JPY |
78,000 JPY |
| Uo to 2.5kbp |
56,000 JPY |
Approx. 1 month |
112,000 JPY |
Approx. 1.5 month |
| Uo to 3.0kbp |
64,500 JPY |
129,000 JPY |
| Uo to 3.5kbp |
73,000 JPY |
146,000 JPY |
| Uo to 4.0kbp |
81,500 JPY |
163,000 JPY |
| Uo to 5.0kbp |
107,000 JPY |
Approx. 1.5
- 2 months |
214,000 JPY |
Approx. 2
- 2.5 months |
| Uo to 6.0kbp |
124,000 JPY |
248,000 JPY |
| Uo to 7.0kbp |
149,500 JPY |
299,000 JPY |
| Uo to 8.0kbp |
166,500 JPY |
333,000 JPY |
| Uo to 9.0kbp |
192,000 JPY |
384,000 JPY |
| Uo to 10.0kbp |
234,500 JPY |
469,000 JPY |
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* Sample quantity and analysis conditions may affect to delivery. Please ask for the actual
delivery when ordered.
* For analysis of known sequence, delivery may be shortened. Please ask us for details.
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How to order
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How to order |
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Please download the order form, fill the blanks and send it attached to an e-mail to .
After sending the order form, please send your samples, primers, electrophoresis photo and order form to the below address.
Please clearly state as "sequencing samples" on the package.
Attn. Custom sequencing team
Hokkaido System Science Co., Ltd.
2-1, Shinkawa Nishi 2-1, Kita-ku, Sapporo-shi
Hokkaido, 001-0932
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* Please read through the cautions on your order placement.
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Please provide: |
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| DNA sample |
Plasmid: Concentration; 100-300ng/uL or more, Quantity; as needed*1 |
| PCR products: Concentration; 10ng/uL or more, Quantity; as needed*1,2 |
Primer
(Custom primer) |
For any kind of sample: Concentration; 10uM, Quantity; 10uL or more Primer is used for the first analysis.
For universal primers, please select from here.
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| Electrophoresis photo |
Please send a photo which shows the result of a marker and your sample on the same gel with their quantity description. |
| Order sheet |
Please download the form from our web site. |
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*1 Required DNA sample volume is depending on the analysis length. 1-5uL of a sample is
used for one reaction which generates a data for approximately 500 bases. Please send a
sample as much volume as possible.
*2 For PCR product, please make sure to be purified after PCR reaction. For our sample
purification service, please click here for the details.
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For the detailed information, please see at the below links.
- For sample preparation, click here.
- For electrophoresis photos, click here.
- For universal primer list, click here.
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*3 Please read through the notice page before the actual order placement.
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