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Hokkaido System Science Co., Ltd. (HSS) delivers sequence data with very short turnaround and at competitive prices. The sequencing is based on high quality oligo-synthesis technique.
HSS strives to work as each customer's own Sequencing Dept.
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| Sequencing Optional Services |
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For pretreatment of your sequencing samples, various optional services are available from our custom DNA sequencing service.
You just send us your purified genome DNA and whole analyses before sequencing service can be supported.
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1. Primer design
2. PCR amplification
3. Sample purification
- Plasmid extraction
- PCR product purification
4. Cloning
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 Optional Services
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1. Primer design |
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Optimally designed primers for your sequencing analysis can be available based on the provided sequence information.
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PCR amplification primer design
PCR amplification primers can be designed for direct sequencing.
Please provide information about the realm to be analyzed, species, accession No. of your intended gene etc.
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Sequencing primer design
Sequencing primers can be designed based on your sequencing result or flanking sequence information. This service helps your primer walking plan based on certain known sequences.
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Price & Domestic Lead-time
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| Service |
Price (tax exclusive) |
Domestic Lead-time |
| PCR amplification primer design |
7,000 JPY |
Approx. 1 week |
| Sequencing primer design |
2,000 JPY |
2 working days |
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* For large quantity orders, it may take longer for delivery. Please inquire if an order is for more
than 10 primers.
* Please be minded that primer synthesis is charged extra.
* If designed primer gives no good result on PCR amplification or sequencing analysis, design
is redone only once.
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2. PCR amplification |
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This is a service to perform PCR amplification of your required realm as a pretreatment for sequencing analysis.
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Please provide:
Purified genome DNA
Primer set*
Amplification proved PCR protocols
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* Primer design and synthesis services are also available on request.
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Price & Domestic Lead-time
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| Service |
Price (tax exclusive) |
Domestic Lead-time |
| PCR amplification |
7,000 JPY (for 1 realm, 1 sample) |
Pl. inquire |
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* Domestic lead-time may be different depending on the conditions such as primer's
availability, sample quantity and so on. Please inquire in advance.
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3. Sample purification |
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Plasmid extraction
Plasmid extraction service from E.. coli.
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PCR product purification
Column purification: Simplified purification to remove remained primers and dNTP.
Gel extraction purification: Intended product band is to be cut out from a gel.
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Price & Domestic Lead-time
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| Service |
Price (tax exclusive) |
Domestic Lead-time |
| Plasmid extraction |
3,500 JPY |
3 working days |
PCR
product |
Column purification |
3,500 JPY |
| Gel extraction |
7,000 JPY |
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* The above is for one prep.
* Final yield after purification depends on provided sample. In case of order for multiple
analyses with same sample, extra purification may be charged depending on sample
quantity.
* It may take longer for delivery depending on sample quantity and analysis conditions.
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4. Cloning |
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Please click here for details of our cloning service.
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How to order
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How to order |
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Service Combination
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Service Combination examples |
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Optional services can be combined with custom DNA sequencing service and available from various stages on request.
How services can be combined?
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Additional sequencing analysis
"Please analyze more sequence of the realm which is flanked to the result of Single Extension service in downstream/upstream direction!"
Another sequencing analysis including primer design and synthesis is available based on the reported sequencing data.
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Service Flow
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Primer design |
2,000 JPY |
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Primer synthesis |
(differs depending on the length) |
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Single Extension Sequencing |
3,500 JPY |
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Total fee |
5,500 JPY (+ Primer synthesis fee) |
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PCR amplification of a certain known realm + sequencing
"It is required to analyze the sequence of the intended realm using known primer set and PCR protocol reported in scientific papers!"
Experiments from PCR amplification to direct sequencing are available with your purified genome DNA, your primer information and PCR protocol.
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Service Flow
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(Example) Sequencing of 1 realm, 5 samples |
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Primer synthesis |
(differs depending on the length) |
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PCR amplification |
7,000 JPY * 5 samples |
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PCR product purification |
3,500 JPY * 5 samples |
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Direct sequencing (double strands)
Single Extension (3,500 JPY * 2) |
7,000 JPY * 5 samples |
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Total fee |
87,500 JPY + (Primer synthesis fee)
(17,500 JPY/sample) + (Primer synthesis fee)
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* PCR primer design service is also available on request with extra charge.
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Full length analysis of interested gene
"I would like to analyze full length of Exon realm of interested gene, and compare it with reference sequence!"
If you just send your purified genome DNA and your interested gene information such as its accession No., design and synthesis of PCR primers which amplify each Exon are available as well as direct sequencing service.
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Service Flow
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(Example) For 1 sample, 7 Exon realms (less than 500bp for each realm) |
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Primer design |
7,000 JPY * 7 realms |
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Primer synthesis |
(differs depending on the length) |
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PCR amplification |
7,000 JPY * 7 realms |
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PCR product purification |
3,500 JPY * 7 samples |
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Direct sequencing (double strands)
Single Extension (3,500 JPY * 2) |
7,000 JPY * 7 realms |
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Total fee |
171,500 JPY + (primer synthesis fee)
(24,500 JPY/realm) + (primer synthesis fee)
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