Plate Sequencing 96 | Hokkaido System Science Co.,Ltd.

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Single Extension	Primer Walking	Plate Sequencing 96	Sequencing Optional Services
Cautions for Sequencing Service	Sample Preparation	Universal Primer List

Hokkaido System Science Co., Ltd. (HSS) delivers sequence data with very short turnaround and at competitive prices. The sequencing is based on high quality oligo-synthesis technique.
HSS strives to work as each customer's own Sequencing Dept.

Plate Sequencing 96

Plate sequencing 96 is the sequencing service for the samples on a 8 strip tube or 96 well plate basis.
Reasonable pricing for tube/plate basis sequencing, suitable for the analyses with large quantity samples such as EST and Point.

Service Description

Price & Domestic Lead-time

How to order

Service Description

This is the sequencing service for the samples on a 8 strip tube or 96 well plate basis. Cycle sequencing reaction is performed once and the result data will be sent to a client.
A standard sequence data comprise approximately between 500 and 700 bases, however; the actual number of bases may differ depending on the status of the sample.

Please select from the below services on your request.

-	Pre-mix Service:
	One time sequencing service using your pre-mixed template and primer.
-	One-pass Service:
	One time sequencing service with separate template and primer

	This service includes:
	- Sequencing reaction - Sequencer RUN - Data delivery

Reagent:

BigDye Terminator v3.1

Equipment:

3730xl DNA Analyzer

Re-analysis will be performed when faulty data is obtained due to the problem of DNA sequencer, consumable supplies and reagents. For the other reasons, re-sequencing will be deemed as a new order, and the extra analysis fee will be charged. Please be minded that the investigation for the faulty data is not included in the basic service.

Deliverable

Analysis result will be delivered by CD-R.

Data includes;

- Base sequence data (Text file)

- Waveform data (ab1 file)

Price and Domestic lead-time

(tax exclusive)

	Pre-mix		One-pass
Quantity	Price	Domestic lead-time	Price	Domestic lead-time
8 strip tube (8 analyses / order)	9,600 JPY(1,200 JPY / analysis)	Approx. 3 working days after the sample arrival.	12,000 JPY (1,500 JPY / analysis)	Approx. 3 working days after the sample arrival.
0.5 plate (48 analyses / order)	48,000 JPY(1,000 JPY / analysis)	Approx. between 3 and 6 working days after the sample arrival.	57,600 JPY(1,200 JPY / analysis)	Approx. between 3 and 6 working days after the sample arrival.
1 plate (96 analyses / order)	57,600 JPY(600 JPY / analysis)		76,800 JPY(800 JPY / analysis
4 plates (384 analyses / order)	-	-	192,000 JPY(500 JPY / analysis)	Approx. between 7 and 12 working days after the sample arrival.

	*		The 1 plate in the above table means 96 well plate.
	*		Please provide your samples in 8 strip tube, 96 well plate or 384 well plate after preparing the concentration.
	*		Depending on sample quantity, options or analysis conditions, it may take longer for delivery.
	*		The above price is for a single sequencing (single strand).
	*		For the order for samples more than 10 plates, please inquire for price and lead-time.

How to order

		Please download the order form, fill the blanks and send it attached to an e-mail to . After sending the order form, please send your samples, primers, electrophoresis photo and order form to the below address. Please clearly state as "sequencing samples" on the package. Attn. Custom sequencing team Hokkaido System Science Co., Ltd. 2-1, Shinkawa Nishi 2-1, Kita-ku, Sapporo-shi Hokkaido, 001-0932
	* Please make sure that your package will be delivered on working days. Thank you for your understanding. * The client is to bear all the actual costs of shipping. * Please read through the cautions on your order placement.

Please provide:

DNA sample^*1 (for 1well/reaction)	Plasmid: Concentration; 100ng/μL, Amount; 10μL or more
DNA sample^*1 (for 1well/reaction)	PCR products: Concentration; 50ng/μL, Amount; 10μL or more^*2
Primer^*3 (custom primer)	For 1 reaction: Concentration; 32μM, Amount; 5μL or more (For free-of-charge universal primers, please select from here.
Electrophoresis photo^*4	Please send a photo which shows the result of a marker and your sample on the same gel with their quantity description.
Order form	Please download the form from our web site.

^*1 Please prepare the samples with the concentration specified as above, and align them in a
     96 well plate. As the amount used for analysis is same for all samples in one 96 well plate,
     the different concentrations may generate faulty data.
^*2 For PCR products, please make sure to be purified after PCR reaction. For the sample
     purification order to us, please click here for the detail.
^*3 Please use four or less kinds of primers for each one plate.
     When five or more kinds of primers are used, please provide a primer plate respective to
     each sample plate.
^*4 Please make sure to perform an agarose gel electrophoresis to confirm sample conditions
     before sending.
^*5 Please read through the cautions on your order placement.