Agilent aCGH Analysis Service | Hokkaido System Science Co.,Ltd.

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Description

* The microarray makes possible simultaneous and comprehensive analysis covering whole genome because of its high density by in-situ synthesis. Maximum average resolution is approx. 6.4kb.

* The array is highly sensitive and uses a 60-mer oligo.

* The analysis is performed using an optimized version of the protocol and reagents recommended by Agilent Technologies as well as a complete system from Agilent Technologies so that the performance of the equipment is maximized.

* The whole process is performed inside a clean room, which means that influence of dust on data as well as that of ozone on Cy-5 are removed.

* Specialized staffs who had training at Agilent Technologies will provide the service.

* Optional service of genome DNA amplification is also available for small volume samples.

For details on Agilent arrays, contact Agilent Technologies.
Mail address:
cag_sales-na@agilent.com

Click here for information on other products:
cag_sales-na@agilent.com

Click here for the microarray gene list:
http://www.chem.agilent.com/scripts/generic.asp?lpage=5175&indcol=Y&prodcol=Y

Probe base sequence information is available for registered users.
Please inquire for the registration procedure.

Analysis Process Flow

Scope of the Service

Service is available as a total solution package or just for scanning and digitalization of an Agilent microarray.

Please inquire if genome DNA sample is small volume, as analysis may be available by amplifying genome DNA (optional service).

« Microarray format »
The microarray design for each format is as below.

Microarray Format for Genome Analysis

Necessary Sample

	No Amplification	With Amplification (Optional Service)
Sample	Genome DNA
Sample Volume	3µg or more	500ng
Sample Concentration	100 ng/µl or more	50 ng/µl or more
OD260/280	1.8 - 2.0	1.8 - 2.0
OD260/230	2.0 or more	2.0 or more

	Sample is to be diluted in nuclease free water.
	Please make sure that the sample genome DNA is not fragmented by using electrophoresis. (Please make sure to attach the electrophoresis image to the sample.)
	Please inquire when the quantity of sample is less than the requirement above.
	Sample is subject to quality control upon receipt by electrophoresis. Analysis might be suspended or new sample requested if the DNA sample shows fragmentation. (Re-submitted sample will be quality controlled for free.) Thank you for your understanding.
	Genome DNA extracted from paraffin is not acceptable for the optional service of genome DNA amplification. Thank you for your understanding.

Deliverables

Microarray scan image (TIFF, BMP)

Digitalization data (TXT file)

Aberrant region list (XLS file) output by Agilent CGH Analytics Software

* Data will be delivered in a CD-R. Encryption available on request.

«For Reference»
Encryption algorithm "Rijndael", which is selected as AES (Advanced Encryption Standard) by National Institute of Standards and Technology (NIST) on October 2000, is applied to our service. This is a proven algorithm with 256 bits block and key length, used widely not only in US but in Japan.

* Agilent CGH Analytics Software is available for free for two weeks.

http://www.chem.agilent.com/cag/bsp/products/CGHAnalytics/CGH1_1.asp

Price and Domestic Lead-time

Price and Domestic Lead-Time

(Tax exclusive)

Microarray	Price per analysis	Domestic Lead-Time
Human Genome CGH 105 / Mouse Genome CGH 105	548,000 JPY	Approx. one month
Human Genome CGH 244 / Mouse Genome CGH 244	598,000 JPY	Approx. one month

*	Genome DNA quality test (twice incl. sample reshipment) is included in the price. The lead-time may vary by order quantity.

Ordering (Domestic)

Please inquire.

Our address:
Attn. Microarray Team
Hokkaido System Science Co., Ltd.
1-2-1, Shinkawa Nishi 2-jo, Kita-ku, Sapporo-shi
Hokkaido,001-0932
Japan
Tel: +81(11)768-5901 Fax:+81(11)768-5951 e-mail: array@hssnet.co.jp

	When delivering the sample, please make sure to be packed with enough ice bags. Use refrigerated courier service.
	Please make sure that your package will be delivered on working days. Thank you for your understanding.
	HSS will not be responsible for any trouble that may occur during transportation.
	Remnants of sample will not be returned. Thank you for your understanding.

Cautions

	Description and scope of the service are subject to changes without notice.
	Lead-time is calculated from the first day HSS starts to work on the target sample.
	Cancellation of an order will be rejected if received after production start. If cancellation is due to force majeure, the client will be asked to pay the service up to the cancellation point.
	Samples are limited to the P1 level defined in "the Law concerning the Conservation and Sustainable Use of Biological Diversity through Regulations on the Use of Living Modified Organisms" (Cartagena Protocol) issued by Ministry of Education, Culture, Sports, Science and Technology.
	Infective samples such as HCV or HBV are not acceptable. In case of using human clinical sample, please confirm if the informed consent is obtained.
	The result of this custom analysis service should be used for the purpose of investigation. The result should not be used for purposes such as production/quality control of a medical product or food, diagnosis, etc.
	HSS will not be responsible for losses a client may incur as a result of the custom analysis service output.
	HSS will not be responsible for troubles related to industrial property rights or safety whose origin is in the sample submitted by a client or the methods used.
	Request for storage or return of samples is not accepted. Thank you for your understanding.
	A genome DNA sample might be rejected as a result of the quality control for acceptance. If that is the case, the second quality control to be performed on the re-submitted sample, will be at no charge. However, please be aware that the genome DNA quality control will be charged from the third time onward.