Agilent miRNA Microarray Analysis Service | Hokkaido System Science Co.,Ltd.

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This is a service using Agilent Technologies’ microarray with innovatively designed probes and offers microarray data which detect 470 kinds of Human mature miRNAs registered in miRbase Release 9.1 database.

Service Description

•	Highly accurate Microarray by in-situ synthesis is used for this service.
•	This covers 470 kinds of Human mature miRNAs in Sanger miRBASE Release 9.1 database.
•	Highly sensitive array, using innovative probe design and direct end labelling method, enables mature miRNA detection.
•	Detection sensitivity is 0.1amol.
•	Dynamic range more than 5 digits would be acquired.
•	Direct labelling from 100ng total RNA enables experiments with only small volume of samples.
•	Analysis is performed with optimised protocol, reagents and Agilent brand equipments, which are all recommended by Agilent Technologies, so that maximum performance of Agilent microarray can be drawn.
•	All experiments are conducted in our clean room where dusts are exhausted not to effect analysis results.

Analysis Process Flow

Scope of the Service

Service is available as a total solution package or just for scanning and digitalization of Agilent microarray.
Please inquire for detail.

« RNA Quality Control »
Sample is subject to quality control upon receipt. This acceptance quality control is performed by using Agilent Technologies’ Bioanalyzer.

« Analysis »
Total RNA is to be labelled with direct end labelling method, one colour label with Cy3, and analysed.

« Microarray format »
15k × 8 packs format

Necessary sample

RNA sample
Sample:	Total RNA
Sample volume:	ca. 500ng
Sample concentration:	100ng/μl or more
OD260/280:	1.8～2.1
OD260/230:	2.0 or more

« Recommended method of RNA extraction »
Please select from the below three methods for RNA extraction.
• TRIZOL (Invitrogen)
• miRNeasy mini kit (QIAGEN)
• mirVana RNA Isolation kit (ABI)
For extraction, please prepare Total RNA including short strand RNA.

*	Optional service for RNA extraction is also available. This service is performed with “miRNeasy mini kit” by QIAGEN. Please click here for the price.

Deliverables

Basic deliverables for this service are as follows;

•	Microarray scan image (TIF, BMP)
•	Digitalization data (TXT or XLS file)
•	Scatter plot
•	List of co-shifted miRNAs on expression profile (Listed by the ones with the largest Fold Change)
•	Sample quality control by Agilent 2100 Bioanalyzer.
•	Sample measurement result by UV (RNA concentration and purity)
•	Report

* Data will be delivered in a CD-R. Encryption available on request.

«For Reference »
Encryption algorithm "Rijndael", which is selected as AES (Advanced Encryption Standard) by National Institute of Standards and Technology (NIST) on October 2000, is applied to our service. This is a proven algorithm with 256 bits block and key length, used widely not only in US but in Japan.

Price and Domestic Lead-time

Price and Domestic Lead-Time

(Tax exclusive)

Microarray	No. of Analysis	Price	Domestic Lead-Time
Human Mouse Rat	2 analyses	640,000 JPY	Approx. 4 weeks after RNA Quality Control
	3 analyses	900,000 JPY
	4 analyses	1,120,000 JPY
	5 analyses	1,300,000 JPY
	6 analyses	1,440,000 JPY
	7 analyses	1,540,000 JPY
	8 analyses	1,600,000 JPY

Ordering (Domestic)

Please fill the order form and send it to the address below. We will let you know necessary information such as how to send your RNA samples on the receipt of your order form.

Click here for download of order form for Agilent microarray analysis service.

Our address:
Attn. Microarray Team
Hokkaido System Science Co., Ltd.
1-2-1, Shinkawa Nishi 2-jo, Kita-ku, Sapporo-shi
Hokkaido,001-0932
Japan
e-mail: array@hssnet.co.jp

Cautions

•	Description and scope of the service are subject to changes without notice.
•	Lead time is calculated from the first day HSS starts to work on the target sample.
•	Cancellation of an order will be rejected if received after production start. If cancellation is due to force majeure, the client will be asked to pay the service up to the cancellation point.
•	Samples are limited to the P1 level defined in "the Law concerning the Conservation and Sustainable Use of Biological Diversity through Regulations on the Use of Living Modified Organisms" (Cartagena Protocol) issued by Ministry of Education, Culture, Sports, Science and Technology.
•	Infective samples such as HCV or HBV are not acceptable.
•	In case of using human clinical sample, please confirm if the informed consent is obtained.
•	The result of this custom analysis service should be used for the purpose of investigation. The result should not be used for purposes such as production/quality control of a medical product or food, diagnosis, etc.
•	HSS will not be responsible for losses a client may incur as a result of the custom analysis service output.
•	HSS will not be responsible for troubles related to industrial property rights or safety whose origin is in the sample submitted by a client or the methods used.
•	Should an Agilent array analysis service be discontinued, the client will be asked to pay the service up to the discontinuation point, including the RNA quality controls if applicable.
•	When delivering the sample, please make sure that the sample is frozen and is packed with enough Dry Ice. Use refrigerated courier service.
•	Please make sure that your package will be delivered on working days. Thank you for your understanding.
•	HSS will not be responsible for any trouble that may occur during transportation.
•	Remnants of sample will not be returned. Please dispense and send the necessary volume.
•	Sample is to be diluted in nuclease free water. HSS recommends Nuclease free water sold by Invitrogen Corporation.
•	DEPC treated water may inhibit enzymatic reaction. Please dissolve total RNA in nuclease free water.
•	Please make sure that the sample RNA is not fragmented by using electrophoresis.
•	Please inquire when the quantity of sample is less than the requirement above.
•	Analysis might be suspended or new sample requested if the RNA sample shows fragmentation. (Re-sent sample will be quality controlled for free.) Thank you for your understanding.
•	If an RNA sample is rejected as a result of the quality control for acceptance, the second quality control to be performed on the re-submitted sample, will be at no charge. However, please be aware that the RNA quality control will be charged from the third time onward.