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Peptide Antibody Production Kit |
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<Service description> |
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Valued set with total experiments from antigenic site search to polyclonal antibody production. |
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Reduction available for the orders of more than three antibodies. |
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Product Specification |
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Antigenic site search |
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The search service for antigenic site suitable for the provided protein sequence. |
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Hydrophilic / hydrophobic plotting |
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Secondary-structure prediction |
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Prediction of potential sequences to be added phosphoric acid or sugar chain |
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Avoidance from the sequences with production difficulties |
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Homology search for candidate sequences by FASTA (only when requested) |
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Please provide the sequence or accession number.
Domestic lead-time: approx. one week after receiving the sequence.
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| Length: |
Maximum 14 residues, excluding the Cys in the terminus |
| Quantity: |
10mg |
| Purity: |
Immunological Purity (> 50%) |
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| Yield: |
5 mg |
| Carrier protein: |
KLH |
| Conjugation: |
MBS |
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| Antibody production |
2 rabbits, 11 weeks immunization period |
6 times immunization (0w, 2w, 4w, 6w, 8w and 10w)
The immunizing dose is 0.2mg per one immunization.
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3 times test bleeding (0w, 7w and 9w)
Approximately 1 ml of blood serum is sent to the requester. The pre-immunization blood serum will be
delivered together with that taken at 7 weeks post-immunization.)
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Terminal bleed (11w post-immunization): decided after discussing with the client and delivered as anti-serum.
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| One ELISA titration performed on the terminal bleed of each rabbit; and the result will
be sent at a later date. |
Note 1: ELISA screening for test bleeds is optional.
Note 2: Other optional services also available. Please inquire.
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Price and Domestic Lead-time |
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Peptide Antibody Production Kit |
| Price (tax exclusive) |
225,000 JPY / antibody |
| Domestic Lead-time |
Approx. four months |
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* 10% reduction is avairable for orders of more than three antibodies. |
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Rapid Rabbit
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<Service description> |
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Total service from antigenic site search to antibody production with shorter lead-time. |
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Product Specification |
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Antigenic site search |
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The search service for antigenic site suitable for the provided protein sequence. |
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Hydrophilic / hydrophobic plotting |
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Secondary-structure prediction |
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Prediction of potential sequences to be added phosphoric acid or sugar chain |
|
Avoidance from the sequences with production difficulties |
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Homology search for candidate sequences by FASTA (only when requested) |
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Please provide the sequence or accession number.
Domestic lead-time: approx. one week after receiving the sequence.
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| Length: |
Maximum 14 residues, excluding the Cys in the terminus. |
| Quantity: |
20mg |
| Purity: |
Immunological Purity (>50%) |
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| Yield: |
5mg |
| Carrier protein: |
KLH |
| Conjugation: |
MBS |
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| Antibody production |
2 rabbits, 6 weeks immunization period |
6 times immunity (0w, 1w, 2w, 3w, 4w and 5w)
The immunizing dose is 0.2mg per one immunization.
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3 times test bleeding (0w, 4w and 5w)
Approximately 1 ml of blood serum is sent to the requester. The pre-immunization blood
serum will be delivered together with that taken at 4 weeks post-immunization.
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| Terminal bleed (6w post-immunization): decided after
discussing with the client and delivered as anti-serum. |
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One ELISA titration performed on the terminal bleed of each rabbit; and the result will be sent at a later date.
Note 1: ELISA screening for test bleeds is optional.
Note 2: Other optional services also available. Please inquire.
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Price and Domestic Lead-time |
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Rapid Rabbit |
| Price (tax exclusive) |
255,000 JPY / antibody |
| Domestic Lead-time |
Approx. 2.5 months |
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* 10% reduction is avairable for orders of more than three antibodies. |
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Polyclonal Antibody Production Service |
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<Service description> |
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Antibody production service with the provided protein / peptide antigen. |
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Product Specification |
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| Antibody production |
2 rabbits, 9 weeks immunization period |
5 times immunization (0w, 2w, 4w, 6w and 8w)
The immunizing dose is 0.2mg per one immunization.
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3 times test bleeding (0w, 5w and 7w)
Approximately 1 ml of blood serum is sent to the requester. The pre-immunization blood serum
will be delivered together with that taken at 5 weeks post-immunization.
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| Terminal bleed (9w): decided after discussing with
the client and delivered as anti-serum. |
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| Antibody production |
2 rabbits, 11 weeks immunization period |
| 6 times immunization (0w, 2w, 4w, 6w, 8w and 10w) Each immunizing dose is 0.2mg.
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3 times test bleeding (0w, 7w and 9w)
Approximately 1 ml of blood serum is sent to the requester. The pre-immunization blood
serum will be delivered together with that taken at 7 weeks post-immunization.
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| Terminal bleed (11w): decided after discussing with the
client and delivered as anti-serum. |
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Please provide 2mg of protein antigen or 2.4mg of peptide antigen.
* For peptide antigen, please provide the carrier conjugated one.
* Carrier conjugation service is available as an option. Please inquire.
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Price and Domestic Lead-time |
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Protein Antigen |
Peptide Antigen |
| Price (tax exclusive) |
140,000 JPY |
153,000 JPY |
| Domestic Lead-time |
Approx. 2.5 months |
Approx. 3 months |
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Footpad Antibody Production |
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<Service description> |
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Rapid polyclonal antibody production service with small amount of antigen. |
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Product Specification |
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| Antibody production |
2 rabbits, 6 weeks immunization period |
| 2 times immunization (0w and 4w) Each immunizing dose is 0.2mg.
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2 times test bleeding (0w and 5w)
Approximately 1 ml of blood serum is sent to the requester. The pre-immunization blood
serum will be delivered together with that taken at 5 weeks post-immunization.
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| Terminal bleed (6w): decided after discussing with
the client and delivered as anti-serum. |
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Please provide 1mg of protein/peptide antigen.
* For peptide antigen, please provide the carrier conjugated one.
* Carrier conjugation service is available as an option. Please inquire.
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Price and Domestic Lead-time |
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Protein Antigen |
Peptide Antigen |
| Price (tax exclusive) |
140,000 JPY |
153,000 JPY |
| Domestic Lead-time |
Approx. 1.5 months |
Approx. 2 months |
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Multi Antibody Production |
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<Service description> |
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This service includes simultaneous immunizations and antibodies
production for several kinds of antigens (protein or peptide). |
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Product Specification |
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| Antibody production |
2 rabbits, 9 weeks immunization period |
| 5 times immunization (0w, 2w, 4w, 6w and 8w) Each immunizing dose is 0.2mg.
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3 times test bleeding (0w, 5w and 7w)
Approximately 1 ml of blood serum is sent to the requester. The pre-immunization
blood serum will be delivered together with that taken at 5 weeks post-immunization.
|
| Terminal bleed (9w): decided after discussing
with the client and delivered as antiserum. |
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| Antibody production |
2 rabbits, 11 weeks immunization period |
| 6 times immunization (0w, 2w, 4w, 6w, 8w and 10w) Each immunizing dose is 0.2mg.
|
|
3 times test bleeding (0w, 7w and 9w)
Approximately 1 ml of blood serum is sent to the requester.
The pre-immunization blood serum will be delivered together with that
taken at 7 weeks post-immunization.
|
| Terminal bleed (11w): decided after discussing
with the client and delivered as antiserum. |
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Please provide 2mg of protein antigen or 2.4mg of peptide antigen.
* For peptide antigen, please provide a carrier conjugated one.
* Carrier conjugation service is available as an option. Please inquire.
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Price and Domestic Lead-time |
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Protein Antigen |
Peptide Antigen |
| Price (tax exclusive) |
Pl. inquire. |
Pl. inquire. |
| Domestic Lead-time |
Various depend on each order |
Various depend on each order |
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Customized Service |
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Product Specification |
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Custom antibody production service available at the required immunization schedule.
* Please inquire about the antibody production method and the schedule.
* Price quotation will be provided on request.
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Optional Services |
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Product Specification |
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| Service |
Description / Domestic Lead-time |
Price |
| Carrier Conjugation 3mg (KLH, BSA etc.) |
Conjugation service of peptide and carrier protein /approx. 1week |
25,500 |
| Career Conjugation 5mg (KLH, BSA etc.) |
Conjugation service of peptide and carrier protein /approx. 1week |
34,000 |
| ELISA (1 rabbit, for once) |
Titer determination of antiserum by a reaction between serially diluted
antiserum and antigen / 1 week |
8,500 |
| Additional immunization (1 rabbit, for once) |
Additional immunization available |
4,250 |
| Additional test bleeding (1 rabbit, for once) |
Additional test bleeding available |
4,250 |
| Additional breeding (1rabbit, for one day) |
Additional breeding day available |
340 |
| Protein A purification (serum 10ml) |
Purification of IgG using protein A / approx. 2 weeks |
42,500 |
| Affinity purification (serum 10ml) |
Antibody purification using columns with antigen-conjugated supports / approx. 2 weeks |
102,000 |
| Affinity purification (additional sera per 10ml) |
Purification with increased sera amount available |
51,000 |
| Intravenous injection |
Immunization with the provided antigen by intravenous injection. Please provide
the information such as how many times and how long the immunization is required in advance. |
30,000 |
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Required Antigen Level |
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Antigen level required for antibody production |
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Protein antigen |
Peptide antigen |
| Immunization |
Required qty. |
Immunization |
Required qty. |
| Polyclonal antibody production |
5 times |
2.0mg |
6 times |
2.4mg |
| Footpad antibody production |
2 times |
1.0mg |
2 times |
1.0mg |
| Multi antibody production |
5 times |
2.0mg |
6 times |
2.4mg |
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* The above are antigen levels required for antibody production with 2 rabbits.
* For multi antibody production, the above is the quantity required for each antigen.
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Antigen level required for ELISA |
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- between 0.1 and 0.2mg (up to 2 times with 2 rabbits)
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Antigen level required for affinity purification |
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How to order |
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Please inquire for order. After the order is confirmed, please arrange the sample shipment.
Sample Shipping Instructions
Please prepare for the antigen as much as your ordered service requires. Please ship the tubes
in a package cooled with Dry Ice and in a -20 °C courier, and notify the scheduled arrival date so
that the package can be handled timely.
* Please make sure that your package will be delivered on working days. Thank you for your understanding.
* HSS will not be responsible for any trouble that may occur during transportation.
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Cautions |
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(1) |
The reactivity of antiserum is measured by ELISA.
It may not be available for some experiments such as western blotting, immunohistochemical staining and
immunoprecipitation. |
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(2) |
Immunization can be stopped due to the death of rabbit. In that case,
re-immunization will be performed once for free unless antigens are taxicant. |
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(3) |
In case of the above (2), additional antigen will be required to be provided. |
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(4) |
Obtained antiserum should be used only for the purpose of research,
and this should not be used for purposes such as medical practice etc. |
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(5) |
HSS will not be responsible for troubles after the product's delivery. |
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(6) |
Infective antigens are not acceptable. |
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(7) |
In case of using human clinical sample as antigen, it is required
to confirm if the informed consent is obtained, and to prove that the sample is not infective. |
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