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Sensitive Analysis of N-terminal Amino Acid Sequence
Price and Domestic Lead-Time Protein Production
 
Ordering
Product Description
  Sensitive analysis of N-terminal amino acid sequence (protein sequencing) is a method to excise amino acid one by one from N-terminal of protein or peptide (Edman degradation method) to be segregated by HPLC, then to detect by UV to determine amino acid sequence by the time the peak is gained (Chromatogram).
With collaboration from Shiga University of Medical Science, HSS provides sequence analysis service for the protein or peptide samples prepared and shipped by client.

Analysis

1 Amino acid sequence analyzer is Procise 492cLC.
1 Residues possible to be analyzed is 5 to 20 residues (depending on the samples).
1 Up to the 3rd residue, possibility of analysis will be determined. Analysis will be discontinued if it was not possible because of N-terminal block or lack of sample molar mass etc. Data up to 5th residue will be taken.
1 Please note that when data was not processed because of the N-terminal block etc., there will be no analysis retry. Request for the analysis of the same sample will be accepted as a new order.

Deliverables

  Amino acid sequence identified by chromatogram, or comment in case analysis was not possible, together with a raw chromatogram data.

Price and Domestic Lead-Time
  <Price>
Basic Fee 50,000JPY per sample
Including analysis fee up to 5th residue
Analysis Fee 6,000JPY per residue
Charged for analysis of more than 6 residues.
When analysis was discontinued by the judgement that it was not possible, Analysis fee will not be charged.
Sample Preparation Fee
(Immobilization to PVDF membrane)
Please inquire
Other Service Charges Please inquire
  <Domestic Lead-Time>
  Within 2 weeks from sample acceptance. Estimated time of arrival will be informed to the client.
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