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Sensitive analysis
of N-terminal amino acid sequence (protein sequencing)
is a method to excise amino acid one by one from
N-terminal of protein or peptide (Edman degradation
method) to be segregated by HPLC, then to detect
by UV to determine amino acid sequence by the time
the peak is gained (Chromatogram).
With collaboration from Shiga University of Medical Science, HSS provides
sequence analysis service for the protein or peptide
samples prepared and shipped by client.
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Amino acid sequence
analyzer is Procise 492cLC. |
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Residues possible to
be analyzed is 5 to 20 residues (depending on the
samples). |
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Up to the 3rd residue,
possibility of analysis will be determined. Analysis
will be discontinued if it was not possible because
of N-terminal block or lack of sample molar mass
etc. Data up to 5th residue will be taken. |
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Please note that when
data was not processed because of the N-terminal
block etc., there will be no analysis retry. Request
for the analysis of the same sample will be accepted
as a new order. |
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Amino acid sequence identified
by chromatogram, or comment in case analysis was
not possible, together with a raw chromatogram data. |
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Within 2 weeks from sample acceptance.
Estimated time of arrival will be informed to the
client. |