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Products & Services
Research Support
Ordering

Research Support Description
Custom cloning service

Target gene is cloned in a desired vector.
Cloning can be done by using the TA cloning or by using any other cloning vector, pre-processed with a restriction enzyme. Sequencing reaction is performed to make sure the cloned gene is the target gene.

Domestic Lead Time / Price

Please inquire.

Primer design service for Sequencing

Primer for sequencing is designed from any desired gene.
Please inquire if the required primer is for PCR.

Caution: Price and lead time for primer synthesis are not included in this service.


Domestic Lead Time / Price

*Note : The lead time and the price do not include primer synthesis

Service Price Domestic Lead Time (Average)
Sequencing Primer Design 20,000 JPY or more per primer 1 working day or longer

*For order more than 10 primers, lead time and the price shall be quoted.
Please inquire on Sequencing Primer
Please inquire on primer for RT-PCR


Custom Gene synthesis service

Synthesis of a DNA with desired sequence. The synthesized DNA will be plasmid integrated for delivery.
Ordering by accession number accepted.

Domestic Lead Time / Price

Price Domestic Lead Time (Average)
Up to 300bp: 51,000 / 1gene
From 301bp: 170 JPY / 1base
From approx. 4 weeks*

*Domestic Lead Time differs depending on length and sequence. Please inquire for the detail.

Mutagenesis service

Site-directed mutagenesis will be made on the provided plasmid DNA based on the provided information.
Unique protein can be obtained by replacement, deletion or insertion of amino acid. Insertion of tag or restricted enzyme site can be made by this service.

Domestic Lead Time / Price

Service Price Domestic Lead Time (Average)
Mutagenesis service 90,000 JPY / 1site on 1 sample Approx. 4 weeks*

*Delivery will be made as soon as the product completed. Please inquire for further details.

RNA extraction and purification service

RNA is extracted from sample cell or tissue and then, purified. The purified RNA is subject to quantitation and degradation test in the bio-analyzer.

Caution: Please note that cells and tissues related to pathogenic and/or infectious virus will not be accepted.

Domestic Lead Time / Price
Unit: Japanese Yen
Service Basic Fee
(Every 12 Samples)
Service Fee
(Per Sample)
Domestic
Lead- time
Sample Quantity
Cell Tissue
RNA
extraction
Mini 45,000 6,000 A week after
sample arrival
1x105-1x107 -30mg
Midi 45,000 15,000 5x106-1x108 20-250mg
Maxi 45,000 25,000 5x107-5x108 150-1,000mg
Total RNA Purification 30,000 5,000 - -

*RNA extraction service price is for one prep each.

RNA quality test service

A complete RNA is essential when performing a gene expression analysis in a microarray or an RT-PCR. For that, an accurate RNA quality test is critical. Please consider using HSS’ RNA quality test prior to any application or investigation where the quality of the RNA sample is critical.

Equipment used for this service is Agilent Technologies’ Agilent 2100 Bioanalyzer.


Domestic Lead Time / Price
Unit: Japanese Yen
Service Basic Fee
(Every 12 Samples)
Service Fee
(Per Sample)
RNA Quality Test 16,000 3,000

Gene amplification by reverse transcription service

Gene amplification by reverse transcription of the primer designed and synthesized from RNA and gene information. The result to be delivered includes the electrophoresis of the amplified gene. Measurement of gene expression quantity by threshold method is available on request.


Domestic Lead Time / Price

Please inquire.

Extraction of DNA from a paraffin embedded specimen and sequencing

DNA is extracted from a sample embedded in a paraffin block and the amplified gene, subject to sequencing. Successful gene amplification is not guaranteed since the target gene might have been fragmented while being embedded in the paraffin.


Domestic Lead Time / Price

Please inquire.

TOF/MS measurement service: Custom mass spectrometry for synthesized peptides

Custom mass spectrometry service for synthesized peptides by using the MALDI-TO/MS method.
Highly accurate reading (error of less than 1 Dalton in the mass spectrometry of a substance with molecular mass of less than 10000).


Domestic Lead Time / Price

Please inquire.

Service Unit Domestic Lead Time (Average)
Custom peptide molecular weight measurement per sample 30,000 JPY or more
Custom DNA molecular weight measurement per sample 20,000 JPY or more
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