Protocol download
MSDS download: SR , PE , CH
Data1 : Structure of DC-6-14, the principal
component of LipoTrust™
Data2 : Electronography of the former liposomes
in the presence of serum
Data3 : Highly efficient gene expression
of LipoTrust™ SR
in the presence of Serum (1)
Data4 : Highly efficient gene expression
of LipoTrust™ SR
in the presence of Serum (2)
Data5 : Characteristics of each liposome
of LipoTrust™ Set in the absence of Serum
Data6 : Highly efficient gene expression
of LipoTrust™ SR
in in vivo experiment
Data7 : Successful examples of cell transfection
Data1 : Structure of DC-6-14, the principal component
of LipoTrust™
O,O'-ditetradecanoyl-N-(α -trimethylammonioacetyl)diethanolamine
chloride (DC-6-14)
Data2 :Electronography of the former liposomes
in the presence of serum
These pictures show change of liposome form when they are disposed in the medium containing 5% FBS. The nanoparticle structures disappeared in the medium with 15min in the case of Other product, while spherical forms are still retained after one hour with LipoTrust SR.
Data3: Highly efficient gene expression of LipoTrust™ SR
in the presence of Serum (1)
This figure shows Luciferase activity after the transfection of Luciferase gene into HRA cells (Human ovarian tumor cells) using either LipoTrust™ SR or one of several other commercial products in 10% FBS-containing medium. LipoTrust™ SR shows remarkably higher efficient gene expression than the other products.
Data4: Highly efficient gene expression of LipoTrust™ SR
in the presence of Serum (2)
This figure shows the level of LacZ-positive cells after the transfection of LacZ gene into several kinds of human tumor cells by either LipoTrust™ SR or one of several other commercial products in 10% FBS-containing medium. LipoTrust™ SR shows remarkably higher efficient gene expression than the other products.
Data5 : Characteristics of each liposome of LipoTrust™
Set in the absence of Serum
This figure shows Luciferase activity after the transfection
of Luciferase gene into human tumor cells using LipoTrust™ set
in serum-free medium.
LipoTrust™ CH shows highly efficient gene expression in
serum-free medium.
Data6 : Highly efficient gene expression of LipoTrust™
SR in in vivo experiment
| Type of Liposomes |
Relative expression of transfected cells |
|
LipoTrust™ SR
|
1.00 ± 0.11
|
|
Product A
|
0.38 ± 0.26
|
|
Product B
|
0.62 ± 0.21
|
|
Product C
|
0.23 ± 0.23
|
|
5 x 106 mEIIL cells were seeded in abdominal cavity
of nude mouse. After three weeks, 1ml of liposome / CAG-LacZ
gene (20µg) was administered to the abdominal cavity of
the mouse, and on the following day, cells were picked from
the abdominal cavity. This table shows the rate of LacZ-positive
cells of the picked cells. LipoTrust™ SR shows highly
efficient gene expression than the other commercial products.
Data7: Successful examples of cell transfection
|
Cell type
|
Transfection Efficiency
|
Cell type
|
Transfection Efficiency
|
| 293 |
Excellent
|
HRA |
Excellent
|
| 3T3 |
Good
|
Jurkat |
Good
|
| A431 |
Good
|
MDCK |
Good
|
| A549 |
Good
|
mEIIL |
Excellent
|
| B95a |
Good
|
Morris |
Excellent
|
| C33A |
Good
|
Neuro-2A |
Excellent
|
| COS-1 |
Good
|
PC3 |
Good
|
| COS-7 |
Excellent
|
RBL-2H3 |
Excellent
|
| ES |
Excellent
|
Walker-256 |
Excellent
|
| H322 |
Good
|
Mouse Fibroblast |
Excellent
|
| H520 |
Good
|
Mouse Granule Cell (Neuron) |
Excellent
|
| HeLa |
Excellent
|
Calf Vascular Endothelial Cell |
Good
|
| HepG2 |
Good
|
Dorsal Root Ganglion |
Excellent
|
|
•Reference
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Nature Review siRNA delivery 129-138
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Biochim.Biophys.Acta,1467,419-430(2000)
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Phorbol ester-potentiated liposomal transfection to monocytic
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J.Biochem.,128,989-998(2000)
Quantative analysis of correlation between number of nuclear
plasmids and gene expression activity after transfection with
cationic liposomes.
Pharm.Res.,19,377-381(2002)
PU.1 is dominant and HAF-1 supplementary for activation of the
gp91phox promoter in human monocytic PLB-985 cells.
J.Biochem.,131.533-540(2002)
Norepinephrine enhances fibrosis mediated by TGF- in cardiac fibroblast.
Hypertension,40,148-154(2002)
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PHARM TECH JAPAN,19,419-433(2003)
Application of stealth-liposomes to anti-cancer chemotherapy
and gene therapy.
Biotherapy,18,353-360(2004)
Ultrasound enhances liposome-mediated gene transfection.
Ultrasound Sonochemistry,Accepted.
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LipoTrust™ EX Series Data Page 
